Nanobody/VHH Affinity Maturation

Nanobody/VHH Affinity Maturation
(Promise 10-fold affinity improvement in as little as 8 weeks)


Nanobody/VHH affinity is one of the key parameters to determine the efficacy and dosage of antibody drugs. Current methods to improve antibody affinity mainly include point mutation, CDR rearrangement, DNA recombination, etc. Through our professional and effective affinity maturation technology, we can quickly locate the key amino acid positions in the nanobody domain, construct a high-quality library, and then screen high-affinity nanobody/VHH through phage display or yeast display.

Case Study: Nanobody/VHH Affinity Maturation (The VHH affinity has been improved 238-fold after maturation)
 
Kinetic curve of interaction between parent nanobody and antigen
 

 
Kinetic curve of interaction between parent nanobody and antigen
 
Affinity Test Results
 

 

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