Article No.: KTSM1331

Specification: 500 μ L (20 times, 50% nanobody coated Agarose beans)

Binding capacity: every 10 μ L Anti mCherry beans (including suspension) can be combined with 2-4 μ g mCherry

Agarose bead size: 〜 45 μ M (4% cross-linked agarose beads)

Storage solution: 20% ethanol, containing 0.03% NaN3

Storage condition: 4 ℃ (frozen storage prohibited)

Shelf life: 12 months

Applications: Immunoprecipitation (IP), Immunocoprecipitation (CoIP), Chromatin Immunoprecipitation (CHIP), RNA Binding Protein Immunoprecipitation (RIP), Enzyme Activity Detection, Mass Spectrometry, etc.

Anti mCherry Agarose Beads are covalently coupled with high affinity and specificity alpaca anti mCherry single domain nano antibodies using agarose beads as a matrix.

Single Domain Antibody (SdAb), also known as Nanobody or VHH (Variable Domain of Heavy Chain), contains only one polypeptide chain, and is the smallest antibody (~15KD) found today that can specifically bind to antigens. The first single domain antibody was intercepted from the variable region of the camel heavy chain antibody (HcAb). Heavy chain antibodies do not have light chains, but consist of only two heavy chains and bind to antigens through a single variable region on the heavy chain. Compared to traditional antibodies, single domain antibodies have multiple advantages, including high affinity (nM-pM), strong specificity, small molecular weight, and can maintain stability under extreme temperature and pH conditions. In addition, single domain antibodies can be produced with high quality and stability, avoiding the issue of batch differences in traditional antibodies.

MCherry is widely used in protein localization and protein dynamics analysis. Anti mCherry (NB) Agarose Beads provide rapid and effective one-step immunoprecipitation that can specifically isolate mCherry labeled target proteins and their interaction factors from cells and tissues. The isolated products can be used for subsequent biophysical and chemical analysis such as nucleic acid analysis, protein analysis, mass spectrometry analysis, and enzyme biopsy assay.

Advantages of anti mCherry Agarose Beads
 

anti-GFP beads	Conventional antibody coupled agarose beads
Direct use	Steps for binding antibodies to protein A/G lipopolysaccharide beads
No interference from heavy and light chains in downstream applications	Interference from heavy and light chains may occur in downstream applications
Strict cleaning strips can be used to screen interacting molecules	The optimal conditions for IgG action will limit the use of buffer solutions and have poor tolerance to certain reagents
Short incubation time (5-30 minutes)	Long incubation time may affect the screening of low affinity interaction factors in CoIP
Stable quality without batch differences	Batch differences in antibody quality
Well validated and qualitative	Each antibody requires separate validation
Generally, the elution product only contains the target protein and its interaction factors, and nano antibodies will not be eluted	In addition to containing target proteins and their interaction factors in the elution product, the antibodies used will also be eluted, and the antibody proteins may affect subsequent detection of target proteins

Immunoprecipitations of mCherry:

Total extract from E.coli BL21(DE3) expression mCherry. Input (I),Flow though (FT) fraction,Wash (W) fraction and Elution (E) fraction。