Library Construction Service
Service Introduction
AlpalifeBio has rich experiences in the construction of nanobody libraries, and adopts optimized primers to ensure diverse high-quality phage display libraries.
Phage Display Library Generation
Construct diverse nanobody/VHH DNA sequences in phagemids, transform into TG1 competent cells, culture and induce VHH display on phage surface.
Yeast Library Construction
Insert diverse nanobody/VHH DNA sequences into the display plasmid vector containing the protein scaffold-Aga2 gene, and transform the competent yeast to prepare the yeast display library.
AlpalifeBio has rich experiences in the construction of nanobody libraries, and adopts optimized primers to ensure diverse high-quality phage display libraries.
Phage Display Library Generation
Construct diverse nanobody/VHH DNA sequences in phagemids, transform into TG1 competent cells, culture and induce VHH display on phage surface.
Yeast Library Construction
Insert diverse nanobody/VHH DNA sequences into the display plasmid vector containing the protein scaffold-Aga2 gene, and transform the competent yeast to prepare the yeast display library.
Extracted RNA with high quality lays a solid foundation for library construction Optimized primers can ensure VHH library diversity.
Optimized competent preparation method can ensure high conversion rate.
Service Content
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